Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names neutrophil cytosol facto| neutrophil NADPH oxidase factor 1| OTTMUSP00000022112| NADPH oxidase subunit (47kDa)| NADPH oxidase subunit (47 kD)| 47 kDa neutrophil oxidase factor| p47phox| p47| Ncf-1| NOXO2| neutrophil cytosolic factor 1|Ncf1 | |
Usage Summary Immunofluorescence:Expression of the protein seen in the cytoplasm and plasma membrane of NIH3T3 and RAW264.7 cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_035006.3 | |
Blocking Peptide EBP10589 | |
Immunogen Peptide with sequence C-DEAEDPDPNYAGEP, from the internal region of the protein sequence according to NP_035006.3. | |
Peptide Sequence C-DEAEDPDPNYAGEP | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 653361 | |
Mouse Gene ID 17969 | |
Rat Gene ID 114553 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:2000. | |
Western Blot Approx 48kDa band observed in lysates of cell line Daudi and in preliminary testing of Mouse Spleen lysate (calculated MW of 44.7kDa according to Human NP_000256.4. This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-p47phox (mouse) Antibody
$423.00
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