Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names POU3F2|POUF3|POU domain, class 3, transcription factor 2|POU class 3 homeobox 2|OTF7|OCT7|BRN2 | |
Usage Summary Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_005595.2 | |
Blocking Peptide EBP10025 | |
Immunogen Peptide with sequence C-AQSLVQGDYGALQSN, from the internal region (near N Terminus) of the protein sequence according to NP_005595.2. | |
Peptide Sequence C-AQSLVQGDYGALQSN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Cow, Pig | |
Reactive Species Human, Mouse, Rat | |
Human Gene ID 5454 | |
Mouse Gene ID 18992 | |
Rat Gene ID 29588 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3µg/ml. | |
Application Type Pep-ELISA, WB |
Goat Anti-POU3F2 / BRN2 / OCT7 Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |
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